Reversal of Cell Death in the Laboratory: Reversal of Clinical Death in the Emergency Room

Introduction: We subjected unicellular dwarf forms of the organism, Stentor coeruleus, to a toxic environment in order to produce evidence of cell death, apoptosis. In previous studies, cell death initiated a progressive release of a replicate image of the cell. In this report we induced a retraction of the replicate image back into the dead cell. Methods: Deep well slides (n=10) were filled with Stentor containing culture media and allowed to evaporate for 24 hours. Rehydration was achieved by refilling each slide with commercially obtained sterile Stentor media. Within 24 hours each slide was inhabited by mobile dwarf forms. The liquid contents from each of 5 slides was transferred into a small beaker with an aliquot of chlorinate tap water. After mixing, the contents were distributed to 5 deep well slides and sealed with a snap cover, active group. The liquid contents from the other 5 slides without the tap water served as the control group.

Background: A previously described dwarf form of the unicellular organism, Stentor coeruleus has been shown to undergo apoptosis, cell death, when placed in a toxic solution. Within minutes these cells progressively released a replicate image.

Methods: Deep well slides (n=10) were filled with Stentor containing culture media and allowed to evaporate for 24 hours. Each slide was rehydrated with commercially obtained sterile Stentor media. Within 24 hours each slide was inhabited by mobile dwarf forms. The liquid contents from each of 5 slides was transferred into a small beaker and an aliquot of chlorinate tap water. After mixing, the contents were distributed to 5 deep well slides and sealed with a snap cover, active group. The liquid contents from the other 5 slides without the tap water served as the control group.

Results: The cells of the active group lost mobility and became stationary within 8-15 minutes. The stationary cells progressively released a replicate image and when fully formed plate magnets applied across the slides induce a magnetic field (ranging from 60-90 milliGauss) allowing retraction of the replicate back into the apoptotic cell. Droplets from these deep well slides placed in a favorable solution was followed by restoration of the living, mobile state.

Conclusions: In the present study we were able to induce, via a magnetic field, retraction of the fully developed replicate back into the dead cell with the subsequent restoration of the living state in a favorable environment. This sequence of events in living cells is analogous to the Out-of-Body phenomenon described in patients resuscitated from clinical death. The medical implications of these finding are discussed.

Keywords: Stentor coeruleus, Dwarf forms, Cell death, Replicate image, Restoration of the living state.