The Importance of PFGE and Rapd Methods in Determination of Philogenetic Relation among E. Coli Sustains Isolated from Extraintestinal Infection and whose Virulance Factors are Defined

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Basak BEDIR, Aymen Aziz Khalid, et al.

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Published: 1 October 2020 | Article Type :

Abstract

In this study, 155 Escherichia coli strains were isolated to investigatethe association between PFGE and RAPD. The freeze-thaw method was applied for RAPD. The results showed PGFE had a higher discrimination power than RAPD-PCR. With the both methodsjust13(30.9%) samples showed clonal relation, the relation might be a random mutation rather than epidemic since the strains were isolated from the in-patient ofdifferent clinics.The study concluded that RAPD method is more advantageous than PEGE method in terms of the phylotyping and interpretation of obtained results.

Keywords: E.coli , Freeze-thaw, Random mutation, PFGE method, Phylotyping

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Basak BEDIR, Aymen Aziz Khalid, et al.. (2020-10-01). "The Importance of PFGE and Rapd Methods in Determination of Philogenetic Relation among E. Coli Sustains Isolated from Extraintestinal Infection and whose Virulance Factors are Defined." *Volume 3*, 4, 1-3