GS-MS Study, Antimicrobial and Antioxidant Activity of Fixed Oil from Ximenia Americana L. Seeds

The aim of the present study is to investigate the chemical constituents of the Essential Oil from Ximenia americana L. plant Seeds and to evaluate its antioxidant potential and antibacterial activity. Using Soxhlet method to extract the essential oil from Ximenia americana L. plant Seeds. The chemical constituents of Ximenia americana Oil were identified and quantified by GC-MS, where paper disc diffusion assay was employed to evaluate the antibacterial activity and Antioxidant activities were evaluated using 1,1- diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity Twentythree components has been identified. Ten of them are major namely 9-Octadecenoic acid (Z)-, methyl ester (32.40% ), Methyl octadeca-9-yn-11-trans- enoate (13.08%) , 9,12-Octadecadiynoic acid, methyl ester (2.69%) , 15- Tetracosenoic acid, methyl ester, (Z)- (4.99 %) , , 10-Nonadecenoic acid, methyl ester (6.81% ), Hexacosanoic acid, methyl ester (4.12%), Tetracosanoic acid, methyl ester (3.02%), Cyclopropaneoctanoic acid, 2-octyl-, methyl ester (15.93%), Octacosanoic acid, methyl ester2.38%) and cis-10-Nonadecenoic acid, methyl ester (7.32% ). the antibacterial showed showed low inhibitory effect against Pseudomonas aeruginosa (9mm), Bacillus subtilis (10mm), albicans (10mm) and Escherichia coli (11mm) and Candida albicans. The DPPH assay, showed moderate antioxidant potential (49, 0.01 compared with standard 91± 0.01;

Keywords: GC-MS Analysis, Antibacterial and Antioxidant Activities.