The aim of this study was to establish an in vitro model of blast lung injury using shock tube. The study was performed from august of 2012 to May 2014 in PLA general hospital respiratory lab.A549 cells were exposed to 100±10 kilopascal (Kpa) blast wave in a culture plate using a shock tube to mimic the in vitro model of blast lung injury. The morphological change and the Hoechst and PrI stain was observed by the light microscopic and electron microscopy. The apoptosis of cells was detected by flow cytometry. The mRNA expression of the inflammatory cytokines was measured by real-time PCR. Our results demonstrated that A549 cells showed increased cellular injury, cellular apoptosis, uptake of propidium iodide (PrI) and inflammatory cytokines mRNA expression after blast exposure. We conclude that we established a simple, reproducible model of blast lung injury.
Keywords: blast injury, cell injury, A549, in vitro, shock tube.