Preliminary Investigations on Distinct Proteomics Profiles for Different Subtypes of Non-Syndromic Cleft Palate Based on SELDI-TOF-MS

Cleft lip and palate usually affect patients in speech, hearing, appearance and psychology, and they need multidisciplinary care from birth to adulthood. However, it is still difficult to make a clear and early diagnosis of cleft palate during pregnancy. The study of differential proteomics will increase our understanding for the pathogenesis and manifestations of cleft palate, and help find methods to assist in early screening, prevent or even in treating cleft palate. Thirty clinical patients with cleft lip and palate and thirty normal controls were collected, the protein expression between different groups was detected by SELDI-TOF-MS technology, and the differentially expressed proteins were analyzed. In between the cleft palate only (CPO) group and the normal control group, the differently expressed protein peaks observed were 4180.3542 and 5639.2742. In between the CPO group and the complete cleft palate and lip group, the peaks were 3291.2347, 4812.2454, 4969.911 and 7791.0462. And in between the unilateral cleft palate and lip group and the bilateral cleft palate and lip group, the peaks were 2529.6336 and 5069.8178. These results may benefit the research on the etiology and early detection of non-syndromic cleft palate.

Keywords: Non-syndromic cleft palate; SELDI-TOF-MS; Proteomics; Etiology.